12 February 1999 Intestine pH measurements using fluorescence imaging: an in-vivo preliminary study
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Abstract
Measurement of gastrointestinal intramucosal pH has been recognized as an important factor in the detection of hypoxia-induced dysfunctions. However, current pH measurement techniques are limited in terms of time and spatial resolution. A major advance in accurate pH measurement was the development of the ratiometric fluorescent indicator dye, 2',7'-bis(carboxyethyl)-4,5- carboxyfluorescein (BCECF). This study aimed to demonstrate the feasibility of fluorescence imaging technique to measure in vivo the pH of intestine. The intestine was inserted in an optical chamber placed under a microscope. Animals were injected i.v. with the pH-sensitive fluorescent dye BCECF. Fluorescence was visualized by illuminating the intestine alternately at 490 and 470 nm. The emitted fluorescence was directed to an intensified camera. The ratio of emitted fluorescence at excitation wavelengths of 490 and 470 nm was measured, corrected and converted to pH by constructing a calibration curve. The pH controls were performed with a pH microelectrode correlated with venous blood gas sampling. We concluded that accurate pH measurements of rat intestine can be obtained by fluorescence imaging using BCECF. This technology could be easily adapted for endoscopic pH measurement.
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Xavier-Marie Marechal, Serge R. Mordon, Jean-Marie Devoisselle, Sylvie Begu, D. Mathieu, Bruno Buys, Guy Dhelin, Jean Claude Lesage, Remi Neviere, Claude Chopin, "Intestine pH measurements using fluorescence imaging: an in-vivo preliminary study", Proc. SPIE 3567, Optical and Imaging Techniques for Biomonitoring IV, (12 February 1999); doi: 10.1117/12.339182; https://doi.org/10.1117/12.339182
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