Estimation of intracellular pH in argon laser scanning confocal microscopes with a laser line of 488 nm

Jan Slavik; Edvard Lanz

doi:10.1117/12.336822

19 January 1999 Estimation of intracellular pH in argon laser scanning confocal microscopes with a laser line of 488 nm

Jan Slavik, Edvard Lanz

Proceedings Volume 3568, Optical Biopsies and Microscopic Techniques III; (1999) https://doi.org/10.1117/12.336822
Event: BiOS Europe '98, 1998, Stockholm, Sweden

Abstract

The reliable and widely used pH-sensitive indicator BCECF (2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein) can also be employed in a classical confocal laser scanning microscopes using 488 nm argon laser excitation line. The intracellular pH maps based on the fluorescence emission ratio tally with those obtained by other techniques.

Citation Download Citation

Jan Slavik and Edvard Lanz "Estimation of intracellular pH in argon laser scanning confocal microscopes with a laser line of 488 nm", Proc. SPIE 3568, Optical Biopsies and Microscopic Techniques III, (19 January 1999); https://doi.org/10.1117/12.336822

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