Optical tomography of living cells using a phase-shifting Linnik microscope

Gennady N. Vishnyakov; Gennady G. Levin

doi:10.1117/12.336834

19 January 1999 Optical tomography of living cells using a phase-shifting Linnik microscope

Gennady N. Vishnyakov, Gennady G. Levin

Proceedings Volume 3568, Optical Biopsies and Microscopic Techniques III; (1999) https://doi.org/10.1117/12.336834
Event: BiOS Europe '98, 1998, Stockholm, Sweden

Abstract

To obtain three-dimensional refractive index or density spatial distribution inside optically transparent living cells, it is necessary to combine computed tomography and interference microscopy. These methods use the oblique illumination and the sample viewing angles scanning for recording a number of the projections. A new application Linnik microscope reflected type for obtaining a high quality phase projections with spatially incoherent laser illumination is described. Phase-shifting technique for automated interferogram analysis is used. Interferometric computed- tomography Linnik laser microscope are fully automated integrated systems that incorporate CCD camera, frame grabber, computed operated PZT mirror as well as intelligible software for interferogram processing, projections preprocessing and tomographic reconstruction. Microscope measurement system and performance characterization are presented. There are some features in angle scanning, projections proceeding, and reconstruction for tomographic investigation of the phase samples that are placed near the mirror surface. For plane mirror the projections viewing angle range may be limited to 90-degree and the investigation object may be represented as a sum of the original object and its mirror reflection. The iterative algorithms for limited-angle tomographic reconstruction were used. Three-dimensional images of living blood cells (lymphocytes) were obtained.

Citation Download Citation

Gennady N. Vishnyakov and Gennady G. Levin "Optical tomography of living cells using a phase-shifting Linnik microscope", Proc. SPIE 3568, Optical Biopsies and Microscopic Techniques III, (19 January 1999); https://doi.org/10.1117/12.336834

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