Phagocytosis: studies by optical tweezers and time-resolved microspectrofluorometry

Herbert Schneckenburger; Reinhard Sailer; Anita Hendinger; Michael H. Gschwend; Manfred Bauer; Wolfgang S. L. Strauss

doi:10.1117/12.336829

19 January 1999 Phagocytosis: studies by optical tweezers and time-resolved microspectrofluorometry

Herbert Schneckenburger, Reinhard Sailer, Anita Hendinger, Michael H. Gschwend, Manfred Bauer, Wolfgang S. L. Strauss

Proceedings Volume 3568, Optical Biopsies and Microscopic Techniques III; (1999) https://doi.org/10.1117/12.336829
Event: BiOS Europe '98, 1998, Stockholm, Sweden

Abstract

Cellular uptake of transparent Latex particles by J774A.1 mouse macrophages has been studied: First, single beads were kept within an optical light trap and located in close vicinity to individual cells. Uptake of the beads was visualized, and intrinsic fluorescence was detected in the spectral range of 420 - 530 nm. Second, time-gated fluorescence spectra of single cells were recorded at pre- selected times during one hour after cellular uptake. A rapid increase of autofluorescence and a subsequent decrease to the level of control cells within about 10 min. was measured within a time gate of 0 - 5 ns after the exciting laser pulses, and attributed to the 'free' coenzyme NAD(P)H. In contrast, fluorescence increase of NAD(P)H bound to proteins (measured within time gates of 5 - 10 ns or 10 - 15 ns) was less pronounced, and the subsequent decrease occurred within a longer period (about one hour).

Citation Download Citation

Herbert Schneckenburger, Reinhard Sailer, Anita Hendinger, Michael H. Gschwend, Manfred Bauer, and Wolfgang S. L. Strauss "Phagocytosis: studies by optical tweezers and time-resolved microspectrofluorometry", Proc. SPIE 3568, Optical Biopsies and Microscopic Techniques III, (19 January 1999); https://doi.org/10.1117/12.336829

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