19 January 1999 Physical modeling of tissue fluorescence: phantom development
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When spectrofluorimetry is applied to the problem of diagnosis, correlation techniques relating spectral features to biotissue status should be used. But the coefficients of correlation equation should account for the relationship of spectral parameters with biochemical and morphological changes associated with the pathology. As a search for these dependencies in actual biotissue is difficult, we offer to employ tissue phantoms -- the physical models mimicking, under conditions of measurement, optical characteristics of the natural object. To model human cervix tissue, we used a three- layer planar structure, with upper 0.3 - 1.0 mm layer simulating epithelium, middle 0.03 - 0.1 mm layer representing basal membrane, and greater than 1.0 mm lower layer modeling subepithelial tissue. As a mechanical base of the structure we used 10% (per weight) gelatin gel. To simulate light scattering by biotissue, the nonabsorbing and nonluminiscent scatters were added to the upper and lower layers. NADH, FAD, and Protoporphyrin IX were added to upper layer. Collagen, as dried thin gelatin film, modeled basal membrane. To reproduce modulation of autofluorescence spectrum by reabsorption within the tissue, we added solution of human hemoglobin to the lower layer. Spectrofluorimetric measurement was performed using various excitation wavelengths (337 nm, 365 plus or minus 20 nm, and 405 plus or minus 20 nm).
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Svetlana P. Chernova, Svetlana P. Chernova, Alexander B. Pravdin, Alexander B. Pravdin, Yurii P. Sinichkin, Yurii P. Sinichkin, Valery V. Tuchin, Valery V. Tuchin, Sandor G. Vari, Sandor G. Vari, "Physical modeling of tissue fluorescence: phantom development", Proc. SPIE 3568, Optical Biopsies and Microscopic Techniques III, (19 January 1999); doi: 10.1117/12.336819; https://doi.org/10.1117/12.336819

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