Selective detection of mitochondrial malfunction in situ by energy transfer spectroscopy

Herbert Schneckenburger; Michael H. Gschwend; Reinhard Sailer; Wolfgang S. L. Strauss; Lars Schoch; Alexander Schuh; Karl Stock; Rudolf W. Steiner; Peter Zipfl

doi:10.1117/12.336827

19 January 1999 Selective detection of mitochondrial malfunction in situ by energy transfer spectroscopy

Herbert Schneckenburger, Michael H. Gschwend, Reinhard Sailer, Wolfgang S. L. Strauss, Lars Schoch, Alexander Schuh, Karl Stock, Rudolf W. Steiner, Peter Zipfl

Author Affiliations +

Proceedings Volume 3568, Optical Biopsies and Microscopic Techniques III; (1999) https://doi.org/10.1117/12.336827
Event: BiOS Europe '98, 1998, Stockholm, Sweden

Abstract

To establish optical in situ detection of mitochondrial malfunction, non-radiative energy transfer from the coenzyme NADH to the mitochondrial marker rhodamine 123 (R123) was examined. Dual excitation of R123 via energy transfer from excited NADH molecules as well as by direct absorption of light results in two fluorescence signals whose ratio is a measure of mitochondrial NADH. These signals are detected simultaneously using a time-gated (nanosecond) technique for energy transfer measurements and a frequency selective technique for direct excitation and fluorescence monitoring of R123. Optical and electronic components of the experimental setup are described and compared with a previously established microscopic system.

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Herbert Schneckenburger, Michael H. Gschwend, Reinhard Sailer, Wolfgang S. L. Strauss, Lars Schoch, Alexander Schuh, Karl Stock, Rudolf W. Steiner, and Peter Zipfl "Selective detection of mitochondrial malfunction in situ by energy transfer spectroscopy", Proc. SPIE 3568, Optical Biopsies and Microscopic Techniques III, (19 January 1999); https://doi.org/10.1117/12.336827

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