17 May 1999 Native fluorescence characteristics of blood plasma during rat liver regeneration
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Proceedings Volume 3599, Optical Diagnostics of Biological Fluids IV; (1999) https://doi.org/10.1117/12.348376
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States
Abstract
Native fluorescence characteristics of blood plasma was studied in the visible region, during the regenerating phase of the rat liver tissue. Animals were subjected to partial hepatectomy (PH). Blood samples were collected after several intervals of post PH time and also from control animals. Native fluorescence spectra of blood plasma were measured at 405 nm excitation. In addition to the primary emission peak around 440 nm, the fluorescence spectra of experimental group of animals showed distinct secondary emission peak around 620 nm, which is found to be absent in the case of control animals. This may be attributed to the presence of endogenous porphyrins. The fluorescence intensity at 620 nm was found to be maximum at about 16 hrs of post PH time and it decreased thereafter with increasing post PH time. The spectral differences between controls and experimental animals were found to be minimal at 240 hrs of post PH time.
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Singaravelu Ganesan, S. Madhuri, V. Anuradha, A. Namasivayam, Diagaradjane Parmeswearan, "Native fluorescence characteristics of blood plasma during rat liver regeneration", Proc. SPIE 3599, Optical Diagnostics of Biological Fluids IV, (17 May 1999); doi: 10.1117/12.348376; https://doi.org/10.1117/12.348376
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