Classification of aortic atherosclerotic lesions with time-resolved fluorescence spectroscopy

Jean-Michel I. Maarek; Laura Marcu; Warren S. Grundfest; Michael C. Fishbein

doi:10.1117/12.351030

2 July 1999 Classification of aortic atherosclerotic lesions with time-resolved fluorescence spectroscopy

Jean-Michel I. Maarek; Laura Marcu; Warren S. Grundfest; Michael C. Fishbein;

Author Affiliations +

Proceedings Volume 3600, Biomedical Imaging: Reporters, Dyes, and Instrumentation; (1999); doi: 10.1117/12.351030
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

In this study, we examine the possibility of differentiating between classes of atherosclerotic lesions based on time- resolved fluorescence spectroscopy and we compare the performance of classification schemes that use either the time-resolved spectra or only the intensity spectra. Transient fluorescence emissions induced by pulsed nitrogen laser excitation was measured on 87 excised samples of human aorta. The samples were classified histologically using the AHA classification Predictor variables derived from the time-resolved spectra included the spectral intensities at 360-510 nm and parameters of a biexponential fit of the fluorescence impulse response function. Stepwise discriminant analysis using these predict variables showed that a few predictor variables sufficed to correctly classify 89 percent of the samples. Excluding the time- dependent decay and using only the spectral intensities, the percentage of correctly classified cases was significantly lower: 51 percent. These results establish that time- resolved fluorescence spectroscopy markedly improved on the performance of steady-state fluorescence spectroscopy for fine classification of atherosclerotic lesions.

Citation Download Citation

Jean-Michel I. Maarek, Laura Marcu, Warren S. Grundfest, Michael C. Fishbein, "Classification of aortic atherosclerotic lesions with time-resolved fluorescence spectroscopy", Proc. SPIE 3600, Biomedical Imaging: Reporters, Dyes, and Instrumentation, (2 July 1999); doi: 10.1117/12.351030; https://doi.org/10.1117/12.351030

ACCESS THE FULL ARTICLE

PERSONAL SIGN IN
Full access may be available with your subscription

Email or Username Forgot your username?

Password Forgot your password?

Show

Remember Email/Username on this computer

Remember Password

No SPIE account? Create an account
or Institutional Sign In via Shibboleth

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $14.40

Non-members: $18.00 ADD TO CART

Show All Keywords

Keywords/Phrases

Search In:

Publication Years