Fluorescence imaging of the propagation of excitability in gastrointestinal muscles

Nelson G. Publicover; Terence K. Smith; Randel J. Stevens

doi:10.1117/12.351010

2 July 1999 Fluorescence imaging of the propagation of excitability in gastrointestinal muscles

Nelson G. Publicover, Terence K. Smith, Randel J. Stevens

Proceedings Volume 3600, Biomedical Imaging: Reporters, Dyes, and Instrumentation; (1999) https://doi.org/10.1117/12.351010
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

Fluorescence imaging is a useful tool to study the sequence an dynamics of the spread of excitability in biological tissues. Gastrointestinal muscles are particularly amenable to imaging using standard video rates because the frequency of events i slow and propagation velocities are slow. Calcium-sensitive fluorescent indicators such as fluo-3 provide effective markers of excitability because optically they exhibit high quantum yields and calcium plays important biological roles including regulating intracellular signaling and muscle contraction. Video sequences of gastrointestinal tissues demonstrate the existence of multiple preferred locations to indicate excitability. The spatial and temporal resolution of microscope-based imagin system allows pacing sites to be identified within single muscle bundles. Anisotropic conduction velocities result in spatially complex patterns of excitability where the range of propagation appears to be limited by 'collisions' with neighboring excitable events or recently activated regions. Although standard video rates are generally not sufficient to monitor more rapid excitable event such as nerve action potentials, fluorescence imaging can be used to investigate excitability mechanisms in tissues such as smooth muscles where event frequencies and propagation velocities are low.

Citation Download Citation

Nelson G. Publicover, Terence K. Smith, and Randel J. Stevens "Fluorescence imaging of the propagation of excitability in gastrointestinal muscles", Proc. SPIE 3600, Biomedical Imaging: Reporters, Dyes, and Instrumentation, (2 July 1999); https://doi.org/10.1117/12.351010

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