2 July 1999 Fluorescent imaging of cells and nucleic acids in bioelectronic chips
Author Affiliations +
Proceedings Volume 3600, Biomedical Imaging: Reporters, Dyes, and Instrumentation; (1999) https://doi.org/10.1117/12.351041
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States
We have performed separation of bacterial and cultured cancer cells from peripheral human blood in microfabricated electronic chips by dielectrophoresis. The isolated cells were examined by staining the nuclei with fluorescent dye followed by laser induced fluorescence imaging. We have also related DNA and RNA from the isolated cells electrically and detected specific marker sequences by DNA amplification followed by electronic hybridization to immobilized capture probes. Efforts toward the construction of a 'laboratory-on- a-chip' system will be presented which involves the selection of DNA probes, dyes, reagents and prototyping of the fully integrated portable instrument.
© (1999) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Jing Cheng, Jing Cheng, Lei Wu, Lei Wu, Jonathan Diver, Jonathan Diver, Paul N. Swanson, Paul N. Swanson, Adam Uribe, Adam Uribe, Michael Krihak, Michael Krihak, Jainamma Krotz, Jainamma Krotz, Kenneth A. Bloch, Kenneth A. Bloch, Bill Street, Bill Street, Trevor Young, Trevor Young, Tony Llevares, Tony Llevares, Tim LeClair, Tim LeClair, Donald E. Ackley, Donald E. Ackley, Michael J. Heller, Michael J. Heller, James P. O'Connell, James P. O'Connell, Tina S. Nova, Tina S. Nova, } "Fluorescent imaging of cells and nucleic acids in bioelectronic chips", Proc. SPIE 3600, Biomedical Imaging: Reporters, Dyes, and Instrumentation, (2 July 1999); doi: 10.1117/12.351041; https://doi.org/10.1117/12.351041


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