Transferrin-mediated tumor delivery of contrast media for optical imaging and magnetic resonance imaging

Andreas Becker; Kai Licha; Mayk Kresse; Bjoern Riefke; Uwe Sukowski; Bernd Ebert; Herbert H. Rinneberg; Wolfhard Semmler

doi:10.1117/12.351024

2 July 1999 Transferrin-mediated tumor delivery of contrast media for optical imaging and magnetic resonance imaging

Andreas Becker, Kai Licha, Mayk Kresse, Bjoern Riefke, Uwe Sukowski, Bernd Ebert, Herbert H. Rinneberg, Wolfhard Semmler

Author Affiliations +

Proceedings Volume 3600, Biomedical Imaging: Reporters, Dyes, and Instrumentation; (1999) https://doi.org/10.1117/12.351024
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

Many tumor cells are characterized by the overexpression of highly specific transferrin receptors. Transferrin is bound to its receptor, which is followed by rapid internalization into intracellular compartments; it can thus be used for the specific delivery of coupled contrast agents in to tumor cells. The aim of our study was to investigate the tumor- specificity of transferrin-modified contrast agents for optical imaging and magnetic resonance imaging (MRI). Indotricarbocyanine, a near-IR absorbing dye, and ultrasmall superparamagnetic iron oxide particles, a contrast material for MRI, were covalently coupled to transferrin and examined in vitro and in vivo for their potential as tumor-specific contrast agents. CEll culture experiments using HT29 and A431 tumor cells showed specific uptake of transferrin- modified contrast agents by the cells at 37 degrees C, while uptake was inhibited at 4 degrees C or in the presence of an excess of unlabeled transferrin. Optical imaging of tumor- bearing nude mice after intravenous injection of transferrin-ICC resulted in pronounced tumor fluorescence. Tumor specificity was also observe in MRI, where transferrin-USPIO accumulated in SMT2A tumor-bearing rats. We conclude that transferrin-modified contrast agents for optical imaging and MRI can be specifically delivered to tumor tissue by use of transferrin receptor pathways.

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Andreas Becker, Kai Licha, Mayk Kresse, Bjoern Riefke, Uwe Sukowski, Bernd Ebert, Herbert H. Rinneberg, and Wolfhard Semmler "Transferrin-mediated tumor delivery of contrast media for optical imaging and magnetic resonance imaging", Proc. SPIE 3600, Biomedical Imaging: Reporters, Dyes, and Instrumentation, (2 July 1999); https://doi.org/10.1117/12.351024

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