Comparison between a conventional epifluorescence microscope and a new highly efficient evanescent wave detector in single-molecule spectroscopic applications

Joerg Enderlein; Thomas Ruckstuhl; Frank Loescher; Martin Boehmer; Stefan Seeger

doi:10.1117/12.347511

3 May 1999 Comparison between a conventional epifluorescence microscope and a new highly efficient evanescent wave detector in single-molecule spectroscopic applications

Joerg Enderlein, Thomas Ruckstuhl, Frank Loescher, Martin Boehmer, Stefan Seeger

Author Affiliations +

Proceedings Volume 3602, Advances in Fluorescence Sensing Technology IV; (1999) https://doi.org/10.1117/12.347511
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

We present a comparison between two basically different optical detection systems: a confocal epifluorescence microscope, and a new evanescent wave detection system employing a parabolic optical element. In a microscope set-up, fluorescence light is collected within a cone around the optical axis, whereas in the evanescent light detector, fluorescence light is collected mainly at angles larger than the so-called critical angle of total internal reflection. Based on a thorough theoretical modeling of both experimental set-ups, comparison between the two detection systems is made Particularly, the optical detection efficiency is compared. 5

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Joerg Enderlein, Thomas Ruckstuhl, Frank Loescher, Martin Boehmer, and Stefan Seeger "Comparison between a conventional epifluorescence microscope and a new highly efficient evanescent wave detector in single-molecule spectroscopic applications", Proc. SPIE 3602, Advances in Fluorescence Sensing Technology IV, (3 May 1999); https://doi.org/10.1117/12.347511

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