3 May 1999 Evaluation of a red fluorescent dye for high-throughput compound screening applications in pharmaceutical discovery research
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Proceedings Volume 3602, Advances in Fluorescence Sensing Technology IV; (1999) https://doi.org/10.1117/12.347522
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States
Abstract
The possibility and potential benefits of using an extreme red fluorescent dye such as Cy5.5TM to label drug discovery target proteins was studied experimentally. Cy5.5 labeled BSA, GFP, and CRP were used as examples of protein-dye conjugates whose binding to corresponding antibody was detected by changes in either rotational or transnational diffusion properties, that is, by either fluorescence polarization or confocal fluorescence correlation spectroscopy (FCS). In addition, FCS was used to quantitate excitation of Cy5.5 to its triplet state. Fluorescence polarization and lifetime were measured as a function of excitation wavelength or glycerol concentration and solvent viscosity.
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Kerry M. Swift, Kerry M. Swift, Edmund D. Matayoshi, Edmund D. Matayoshi, "Evaluation of a red fluorescent dye for high-throughput compound screening applications in pharmaceutical discovery research", Proc. SPIE 3602, Advances in Fluorescence Sensing Technology IV, (3 May 1999); doi: 10.1117/12.347522; https://doi.org/10.1117/12.347522
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