Fluorescence decay kinetics and localization of disulphonated aluminium phthalocyanine in fibroblasts: a confocal fluorescence microscopy study

Zdenek Petrasek; Richard B. Ostler; Ilya V. Eigenbrot; David Phillips

doi:10.1117/12.347547

3 May 1999 Fluorescence decay kinetics and localization of disulphonated aluminium phthalocyanine in fibroblasts: a confocal fluorescence microscopy study

Zdenek Petrasek, Richard B. Ostler, Ilya V. Eigenbrot, David Phillips

Author Affiliations +

Proceedings Volume 3602, Advances in Fluorescence Sensing Technology IV; (1999) https://doi.org/10.1117/12.347547
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

Steady state and time resolved confocal fluorescence microscopy, using a point scanning system, is applied to an investigation of the early stages of photo-induced changes in 3T3-L1 murine fibroblasts using di-sulphonated aluminum phthalocyanine (AlPcS₂) as a photosensitizer. A comparison is made with data obtained using a line scan system and V79-4 Chinese hamster fibroblasts. The steady state data obtained in this work demonstrate that intracellular AlPcS₂ fluorescence intensity increases progressively on photoirradiation. Time-resolved studies indicate that this could result from a progressive decrease in the concentration of the self-quenched membrane-associated form of AlPcS₂ following its conversion into the fluorescent monomeric form.

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Zdenek Petrasek, Richard B. Ostler, Ilya V. Eigenbrot, and David Phillips "Fluorescence decay kinetics and localization of disulphonated aluminium phthalocyanine in fibroblasts: a confocal fluorescence microscopy study", Proc. SPIE 3602, Advances in Fluorescence Sensing Technology IV, (3 May 1999); https://doi.org/10.1117/12.347547

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