3 May 1999 Sensitive fiber optics-based system for real-time detection of PCR-amplified DNA using molecular beacons
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Proceedings Volume 3602, Advances in Fluorescence Sensing Technology IV; (1999) https://doi.org/10.1117/12.347540
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States
Molecular Beacon hairpin shaped fluorescent oligonucleotide probes are powerful tools for quantifying specific nucleic acid sequences. Stratagene is developing a sensitive system, using these probes, for detecting and quantifying initial template copy number of nucleic acid sequences in real time during PCR amplification. The system allows parallel multiple fluorophore detection for many applications including allele discrimination and quantitative gene expression analysis. This instrument, combined with Stratagene's Sentinel Molecular Beacon kits, provides an effective system for molecular biology research. We report here the design and utility of an instrument that combines the capabilities of a microplate fluorescence reader with a PCR thermocycler into a low cost real time detection system. The instrument integrates a multiple fluorophore parallel fiber optic excitation and emission detection system, a precision X-Y translation stage, and a high performance thermoelectric temperature cycler with a computer controlled data collection and analysis system. The system uses standard PCR tubes, tube strips, and 96 well plates as the sample format. The result is a low cost, reliable, and easy to use system with premium performance for nucleic acid quantification in real time. 10
© (1999) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Taylor A. Reid, Michelle Cayouette, Larry Brown, Ali Reza Mousavi, John Slaney, Jane Moores, "Sensitive fiber optics-based system for real-time detection of PCR-amplified DNA using molecular beacons", Proc. SPIE 3602, Advances in Fluorescence Sensing Technology IV, (3 May 1999); doi: 10.1117/12.347540; https://doi.org/10.1117/12.347540


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