Simple scanning near-infrared time-correlated single-photon counting instrument with a pulsed diode laser and avalanche photodiode (APD) for time-resolved measurements in scanning applications with

Yuling Zhang; Steven A. Soper; Lyle Richard Middendorf; John A. Wrum; Rainer Erdmann; Michael Wahl

doi:10.1117/12.347539

3 May 1999 Simple scanning near-infrared time-correlated single-photon counting instrument with a pulsed diode laser and avalanche photodiode (APD) for time-resolved measurements in scanning applications with a DNA sequencer

Yuling Zhang, Steven A. Soper, Lyle Richard Middendorf, John A. Wrum, Rainer Erdmann, Michael Wahl

Author Affiliations +

Proceedings Volume 3602, Advances in Fluorescence Sensing Technology IV; (1999) https://doi.org/10.1117/12.347539
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

A simple apparatus for time-correlated single photon counting (TCSPC) measurements in the near-infrared (near-IR) region for scanning-type applications has been constructed and examined. The apparatus consisted of five major components including a pulsed diode laser source (lasing wavelength equals 780 nm; repetition rate equals 80 MHz; power equals 5 mW; pulse width equals 150 ps), an integrated microscope, a large photoactive area avalanche photodiode (APD), a TCSPC PC-board including the electronics and a windows-based software package for accumulating the fluorescence decay profiles. The instrument response function of this assembly was found to be 460 ps, which is adequate for measuring lifetimes with (tau) _f greater than or equal to 500 ps. Due to the small size of the device, it also allowed implementation into scanning experiments where lifetimes were measured. To demonstrate this capability, a three-well microscope slide containing a near-IR dye was scanned. The decay profile of the near-IR dye, aluminum 2,3-naphthalocyanine, was collected and analyzed to obtain its lifetime, which was found to be 2.73 ns, in close agreement to literature values for this particular dye. In addition, a three dimensional image of aluminum 2,3- naphthalocyanine fluorescence decays was acquired by scanning the microscope head over this three-well glass slide. In the scanning mode, the IRFs as well as the decays of the dyes were found to be very stable. The device demonstrated a concentration detection sensitivity of 2.33 nM, however, the dynamic range was limited due to the APD and its slow time constant (passive quenching). In addition, this microscope head was installed in a LI-COR DNA Sequencer 4000 for collection of scanning images.

Citation Download Citation

Yuling Zhang, Steven A. Soper, Lyle Richard Middendorf, John A. Wrum, Rainer Erdmann, and Michael Wahl "Simple scanning near-infrared time-correlated single-photon counting instrument with a pulsed diode laser and avalanche photodiode (APD) for time-resolved measurements in scanning applications with a DNA sequencer", Proc. SPIE 3602, Advances in Fluorescence Sensing Technology IV, (3 May 1999); https://doi.org/10.1117/12.347539

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available