Biological assays in microfabricated structures

Daniel M. Fishman; Thomas L. Fare; Qianping Dong; Z. Hugh Fan; Timothy J. Davis; Rajan Kumar

doi:10.1117/12.346741

21 April 1999 Biological assays in microfabricated structures

Daniel M. Fishman, Thomas L. Fare, Qianping Dong, Z. Hugh Fan, Timothy J. Davis, Rajan Kumar

Proceedings Volume 3603, Systems and Technologies for Clinical Diagnostics and Drug Discovery II; (1999) https://doi.org/10.1117/12.346741
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

Microfluidic control in microfabricated glass channels enables miniaturized, fast, and multianalyte assays. We are applying this technology in several areas, including real- time environmental monitoring for airborne biological agents. Two complementary approaches are being used in parallel. the first is assaying for the presence of nucleotide sequences that are markers for specific hazardous, engineered bacteria. The second is an assay that monitors the functionality of an in vitro biochemical pathway, in which the pathway that is chosen is sensitive to the presence of the class of toxins to be detected. The first approach is discussed here. The detected signal from the nucleic-acid-based assay is from fluorescently labeled probes that hybridize to bead-bound amplified DNA sequences. Detection approaches and their benefits will be discussed.

Citation Download Citation

Daniel M. Fishman, Thomas L. Fare, Qianping Dong, Z. Hugh Fan, Timothy J. Davis, and Rajan Kumar "Biological assays in microfabricated structures", Proc. SPIE 3603, Systems and Technologies for Clinical Diagnostics and Drug Discovery II, (21 April 1999); https://doi.org/10.1117/12.346741

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