Use of a Fluorometric Imaging Plate Reader in high-throughput screening

Duncan R. Groebe; Sujatha Gopalakrishnan; Holly Hahn; Usha Warrior; Linda Traphagen; David J. Burns

doi:10.1117/12.346753

21 April 1999 Use of a Fluorometric Imaging Plate Reader in high-throughput screening

Duncan R. Groebe, Sujatha Gopalakrishnan, Holly Hahn, Usha Warrior, Linda Traphagen, David J. Burns

Proceedings Volume 3603, Systems and Technologies for Clinical Diagnostics and Drug Discovery II; (1999) https://doi.org/10.1117/12.346753
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

High-throughput screening (HTS) efforts at Abbott Laboratories have been greatly facilitated by the use of a Fluorometric Imaging Plate Reader. The FLIPR consists of an incubated cabinet with integrated 96-channel pipettor and fluorometer. An argon laser is used to excite fluorophores in a 96-well microtiter plate and the emitted fluorometer. An argon laser is used to excite fluorophores in a 96-well microtiter plate and the emitted fluorescence is imaged by a cooled CCD camera. The image data is downloaded from the camera and processed to average the signal form each well of the microtiter pate for each time point. The data is presented in real time on the computer screen, facilitating interpretation and trouble-shooting. In addition to fluorescence, the camera can also detect luminescence form firefly luciferase.

Citation Download Citation

Duncan R. Groebe, Sujatha Gopalakrishnan, Holly Hahn, Usha Warrior, Linda Traphagen, and David J. Burns "Use of a Fluorometric Imaging Plate Reader in high-throughput screening", Proc. SPIE 3603, Systems and Technologies for Clinical Diagnostics and Drug Discovery II, (21 April 1999); https://doi.org/10.1117/12.346753

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