1 June 1999 Intracellular gradients of free calcium visualized in sensory and neuronal cells by a high-performance fluorescence imaging system
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Proceedings Volume 3604, Optical Diagnostics of Living Cells II; (1999) https://doi.org/10.1117/12.349212
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States
Abstract
This paper describes results obtained with a novel imaging system based on a fast CCD device. Sensor's output was digitized at 12 bit/pixel by customized electronics, reaching acquisition rates as high as 800 frames/s with a full-frame resolution of 128 X 128 pixels. The software developed for the project permitted the sequential capture of thousands of images directly to host PC RAM without frame loss even at the maximum readout rate (16 MHz). It is shown that the high spatio-temporal resolution of this apparatus is of value when investigating the time-course of rapid intracellular Ca2+ fluorescence transients, particularly those associated with neuronal action potentials near physiological temperature.
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Alberto Colavita, Gabriele Capello, Razaq B. Ijaduola, Antonio Cunei, Laura Lagostena, Marco Canepari, Fabio Mammano, "Intracellular gradients of free calcium visualized in sensory and neuronal cells by a high-performance fluorescence imaging system", Proc. SPIE 3604, Optical Diagnostics of Living Cells II, (1 June 1999); doi: 10.1117/12.349212; https://doi.org/10.1117/12.349212
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