Quantitative phase contrast microscopy of living cells by numerical reconstruction of digital holograms

Etienne Cuche; Pierre Marquet; Pierre J. Magistretti; Christian D. Depeursinge

doi:10.1117/12.349218

1 June 1999 Quantitative phase contrast microscopy of living cells by numerical reconstruction of digital holograms

Etienne Cuche, Pierre Marquet, Pierre J. Magistretti, Christian D. Depeursinge

Proceedings Volume 3604, Optical Diagnostics of Living Cells II; (1999) https://doi.org/10.1117/12.349218
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

We describe a new method of optical microscopy which allows simultaneously intensity and quantitative phase contrast imaging. The method is based on a new holographic imaging technique which involves a CCD camera as recording device and a numerical method for the reconstruction. An application to phase contrast imaging of living astrocytes is presented.

Citation Download Citation

Etienne Cuche, Pierre Marquet, Pierre J. Magistretti, and Christian D. Depeursinge "Quantitative phase contrast microscopy of living cells by numerical reconstruction of digital holograms", Proc. SPIE 3604, Optical Diagnostics of Living Cells II, (1 June 1999); https://doi.org/10.1117/12.349218

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