6 May 1999 Optical sectioning using finite-sized multiple-aperture arrays in fluorescence direct-view microscopy: experimental results
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Proceedings Volume 3605, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VI; (1999) https://doi.org/10.1117/12.347569
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States
Abstract
Confocal fluorescence imaging is widely used, particularly for biological applications, and also notably in direct-view microscopes. Recent work has compared the use of coherent and incoherent illumination sources on the optical sectioning characteristics of fluorescence direct-view microscopes. However this detailed comparison has been done in theory. This paper addresses the experimental aspects of using coherent light sources in fluorescence imaging using a range of finite- sized, multiple-aperture arrays. The experimental difficulties of choosing a suitable uniform, flat, fluorescent plane with a high quantum efficiency are considered. Axial response curves obtained with a fluorescent laser dye sample are presented.
© (1999) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Patrick J. Smith, Patrick J. Smith, Cian M. Taylor, Cian M. Taylor, Eithne M. McCabe, Eithne M. McCabe, "Optical sectioning using finite-sized multiple-aperture arrays in fluorescence direct-view microscopy: experimental results", Proc. SPIE 3605, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VI, (6 May 1999); doi: 10.1117/12.347569; https://doi.org/10.1117/12.347569
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