Detecting exposure to environmental organic toxins in individual cells: toward development of a microfabricated device

Hoi-Ying N. Holman; Miqin Zhang; Regine Goth-Goldstein; Michael C. Martin; Marion Russell; Wayne R. McKinney; Mauro Ferrari; Jennie C. Hunter-Cevera

doi:10.1117/12.350062

3 June 1999 Detecting exposure to environmental organic toxins in individual cells: toward development of a microfabricated device

Hoi-Ying N. Holman, Miqin Zhang, Regine Goth-Goldstein, Michael C. Martin, Marion Russell, Wayne R. McKinney, Mauro Ferrari, Jennie C. Hunter-Cevera

Author Affiliations +

Proceedings Volume 3606, Micro- and Nanofabricated Structures and Devices for Biomedical Environmental Applications II; (1999) https://doi.org/10.1117/12.350062
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

A new method is being developed for quickly screen for the human exposure potential to polycyclic aromatic hydrocarbons (PAHs) and organochlorines (OCs). The development involves two key elements: identifying suitable signals that represent intracellular changes that are specific to PAH and OC exposure, and constructing a device to guide the biological cell growth so that signals from individual cells are consistent and reproducible. We are completing the identification of suitable signals by using synchrotron radiation-based (SR) Fourier-transform infrared (FTIR) spectromicroscopy in the mid-infrared region (4000 - 400 cm^-1). Distinct changes have been observed in the IR spectra after treatment of human cells in culture medium with PAHs and OCs. The potential use of this method for detecting exposure to PAHs and OCs has been tested and compared to a reverse transcription polymerase chain reaction (RT-PCR) assay that quantifies increased expression of the CYP1A1 gene in response to exposure to PAHs or OCs.

Citation Download Citation

Hoi-Ying N. Holman, Miqin Zhang, Regine Goth-Goldstein, Michael C. Martin, Marion Russell, Wayne R. McKinney, Mauro Ferrari, and Jennie C. Hunter-Cevera "Detecting exposure to environmental organic toxins in individual cells: toward development of a microfabricated device", Proc. SPIE 3606, Micro- and Nanofabricated Structures and Devices for Biomedical Environmental Applications II, (3 June 1999); https://doi.org/10.1117/12.350062

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