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17 June 1999 Progress toward imaging biological samples with NSOM
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Proceedings Volume 3607, Scanning and Force Microscopies for Biomedical Applications; (1999)
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States
Advancements in near-field scanning optical microscopy (NSOM) tip design as well as an interferometric feedback mechanism are presented for the common goal of imaging living biological samples under physiological conditions. The ability of a cantilevered tip to track the subtle topography changes of a fragile lipid film in an aqueous environment is demonstrated. In order to further the imaging capabilities, the probes have been chemically etched to reduce the spring constants of the tips, thereby lowering the forces imparted on the sample. An optical feedback mechanism used as an alternative to the conventional force feedback is also described. Utilizing this optical feedback mechanism, images have been obtained of fixed cells underwater. Finally, progress towards modifying the NSOM tip for chemical sensor applications is discussed in the context of eventually measuring ion fluxes through single protein channels. Together these advancements demonstrate the potential of NSOM for studying live cells.
© (1999) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
M. Annie Lee, Chad E. Talley, Sarah A. Vickery, Jeffrey R. Krogmeier, Christopher W. Hollars, Hitoshi Shiku, and Robert C. Dunn "Progress toward imaging biological samples with NSOM", Proc. SPIE 3607, Scanning and Force Microscopies for Biomedical Applications, (17 June 1999);

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