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17 June 1999 Scanning near-field optical/atomic-force microscope (SNOAM) for biomedical applications
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Proceedings Volume 3607, Scanning and Force Microscopies for Biomedical Applications; (1999)
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States
A scanning near-field optical/atomic-force microscope (SNOAM) system was applied to simultaneous topographic and fluorescence imaging of biological samples in air and liquid. The SNOAM uses a bent optical fiber simultaneously as a dynamic mode atomic-force microscopy cantilever and a scanning near-field optical microscopy probe. The SNOAM system used 458 or 488 nm from Ar ion laser multiline of excitation of green fluorescent protein (GFP), since a native GFP has been known to give a maximum at 395 nm and a broad absorption spectrum until 500 nm. Topographic and fluorescence images of recombinant E.coli were obtained simultaneously with a high spatial resolution which was apparently better than that of a conventional confocal microscope.
© (1999) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Eiichi Tamiya, Shinichiro Iwabuchi, Atsuko Hashigasako, Yuji Murakami, Toshifumi Sakaguchi, Yasutaka Morita, and Kenji Yokoyama "Scanning near-field optical/atomic-force microscope (SNOAM) for biomedical applications", Proc. SPIE 3607, Scanning and Force Microscopies for Biomedical Applications, (17 June 1999);

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