Single-virus analysis by correlated optical spectroscopy and atomic force microscopy

Warren C. W. Chan; Shuming Nie

doi:10.1117/12.350617

17 June 1999 Single-virus analysis by correlated optical spectroscopy and atomic force microscopy

Warren C. W. Chan, Shuming Nie

Proceedings Volume 3607, Scanning and Force Microscopies for Biomedical Applications; (1999) https://doi.org/10.1117/12.350617
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

Single adeno- and tobacco mosaic viruses have been studied by correlated fluorescence spectroscopy and tapping-mode atomic force microscopy. The size and shape of spatially isolated, fluorescently tagged viruses are measured on the nanometer scale, and the fluorescent labels in each virus are determined by wavelength-resolved spectroscopy. This work extends ultrasensitive measurement to the single-virus level and is expected to have applications in studying gene therapy vectors and virus-cell interactions.

Citation Download Citation

Warren C. W. Chan and Shuming Nie "Single-virus analysis by correlated optical spectroscopy and atomic force microscopy", Proc. SPIE 3607, Scanning and Force Microscopies for Biomedical Applications, (17 June 1999); https://doi.org/10.1117/12.350617

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