Human coronary atherosclerosis studied by morphological NIR Raman confocal microspectroscopy

Hendrik P. J. Buschman; Jason T. Motz; Maryann Fitzmaurice; Arnoud van der Laarse; Albert V.G. Bruschke; Michael S. Feld

doi:10.1117/12.345400

9 April 1999 Human coronary atherosclerosis studied by morphological NIR Raman confocal microspectroscopy

Hendrik P. J. Buschman, Jason T. Motz, Maryann Fitzmaurice, Arnoud van der Laarse, Albert V.G. Bruschke, Michael S. Feld

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Proceedings Volume 3608, Biomedical Applications of Raman Spectroscopy; (1999) https://doi.org/10.1117/12.345400
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

We used Raman microspectroscopy to study all major morphological structures from normal and atherosclerotic tissue expressing different stages of disease. Thin sections from coronary artery sample;s were studied with a Raman microspectrometer system. Raman spectra were collected from the internal and external elastic lamina, collagen fibers/fibrous cap, foam cells, smooth muscle cells, necrotic core, adventitial fat, (beta) -carotene containing crystal, cholesterol crystals and calcium mineralizations. To assess the chemical composition of the examined morphological structures each spectrum was modeled with a chemical algorithm containing the Raman spectra of the major chemicals present in artery wall. The results of this analysis show that the chemical composition of each morphological structure is well defined and shows little variation between similar structures from different samples.

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Hendrik P. J. Buschman, Jason T. Motz, Maryann Fitzmaurice, Arnoud van der Laarse, Albert V.G. Bruschke, and Michael S. Feld "Human coronary atherosclerosis studied by morphological NIR Raman confocal microspectroscopy", Proc. SPIE 3608, Biomedical Applications of Raman Spectroscopy, (9 April 1999); https://doi.org/10.1117/12.345400

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KEYWORDS

Raman spectroscopy

Arteries

Crystals

Foam

Imaging spectroscopy

Calcium

Tissues

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