Noninvasive assessment of ocular tissue using confocal Raman spectroscopy

James P. Wicksted; Noel J.C. Bauer; Roel J. Erckens; Franciscus H. M. Jongsma; Eefke E. Smit-Kepel; Massoud Motamedi; Wayne F. March

doi:10.1117/12.345392

9 April 1999 Noninvasive assessment of ocular tissue using confocal Raman spectroscopy

James P. Wicksted, Noel J.C. Bauer, Roel J. Erckens, Franciscus H. M. Jongsma, Eefke E. Smit-Kepel, Massoud Motamedi, Wayne F. March

Author Affiliations +

Proceedings Volume 3608, Biomedical Applications of Raman Spectroscopy; (1999) https://doi.org/10.1117/12.345392
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

We have developed a confocal Raman spectroscopy system in order to noninvasively characterize ocular tissue with both an in vitro nd in vivo capability. This systems consists of a long working distance optical probe, which focuses the incident laser light on the tissue and collects the backscattered Raman signal, a single grating spectrometer with CCD camera, and an optical fiber which couples the optical probe to the spectrometer. Essential to the confocal design is that the sample volume is limited, preventing detection of interference signals and fluorescence from adjacent ocular tissues. This sample volume is adjustable by changing the diameter of the collection fiber which acts as the pinhole in the system. Potential applications of this technique such as assessing corneal hydration and quantifying pharmacokinetic drug transport across the cornea will be discussed.

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James P. Wicksted, Noel J.C. Bauer, Roel J. Erckens, Franciscus H. M. Jongsma, Eefke E. Smit-Kepel, Massoud Motamedi, and Wayne F. March "Noninvasive assessment of ocular tissue using confocal Raman spectroscopy", Proc. SPIE 3608, Biomedical Applications of Raman Spectroscopy, (9 April 1999); https://doi.org/10.1117/12.345392

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