Surface-enhanced Raman scattering spectra of TvNPV polyhedrin protein in Ag sol

PeiDi Bao; XinMing Liu; TianQuan Huang; Tieqiao Wu; Shiqui Liu

doi:10.1117/12.345406

9 April 1999 Surface-enhanced Raman scattering spectra of TvNPV polyhedrin protein in Ag sol

PeiDi Bao, XinMing Liu, TianQuan Huang, Tieqiao Wu, Shiqui Liu

Proceedings Volume 3608, Biomedical Applications of Raman Spectroscopy; (1999) https://doi.org/10.1117/12.345406
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

The surface-enhanced Raman Scattering (SERS) spectra of insect virus: the Polyhedrin Protein and Trabala Vishnou Nuclear Polyhedrosis Virus (TvNPV-PP) adsorbed on the surface of silver colloid particles and their Ordinary Raman Scattering (ORS) spectrum were investigated. The SERS spectra and the ORS spectrum of TvNPV-PP show their correlation. In ORS spectrum of TvNPV-PP. The intense amide I at 1656 nm^-1, the medium-intense amide I 1673 cm^-1 and a week amide III at 1280 cm^-1 show that TvNPV-PP has predominantly (alpha) -helical and (beta) - sheet structure. In SERS spectra of TvNPV-PP the intense bands related to carboxylate groups vibrations suggest that TvNPV-PP are adsorbed on the silver colloid surface through the carboxylate groups. The bands of amide I and amide III could not be observed in SERS residues increased their distance from Ag surface and weaken the enhanced effect of the corresponding vibrations. Chemisorption is a main mechanism in the SERS spectra on silver hydrosols for TvNPV- PP and its mechanism of enhancement has a short-range character. The SERS enhancement factor is approximately 10².

Citation Download Citation

PeiDi Bao, XinMing Liu, TianQuan Huang, Tieqiao Wu, and Shiqui Liu "Surface-enhanced Raman scattering spectra of TvNPV polyhedrin protein in Ag sol", Proc. SPIE 3608, Biomedical Applications of Raman Spectroscopy, (9 April 1999); https://doi.org/10.1117/12.345406

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