9 April 1999 Surface-enhanced Raman scattering spectra of TvNPV polyhedrin protein in Ag sol
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Proceedings Volume 3608, Biomedical Applications of Raman Spectroscopy; (1999) https://doi.org/10.1117/12.345406
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States
Abstract
The surface-enhanced Raman Scattering (SERS) spectra of insect virus: the Polyhedrin Protein and Trabala Vishnou Nuclear Polyhedrosis Virus (TvNPV-PP) adsorbed on the surface of silver colloid particles and their Ordinary Raman Scattering (ORS) spectrum were investigated. The SERS spectra and the ORS spectrum of TvNPV-PP show their correlation. In ORS spectrum of TvNPV-PP. The intense amide I at 1656 nm-1, the medium-intense amide I 1673 cm-1 and a week amide III at 1280 cm-1 show that TvNPV-PP has predominantly (alpha) -helical and (beta) - sheet structure. In SERS spectra of TvNPV-PP the intense bands related to carboxylate groups vibrations suggest that TvNPV-PP are adsorbed on the silver colloid surface through the carboxylate groups. The bands of amide I and amide III could not be observed in SERS residues increased their distance from Ag surface and weaken the enhanced effect of the corresponding vibrations. Chemisorption is a main mechanism in the SERS spectra on silver hydrosols for TvNPV- PP and its mechanism of enhancement has a short-range character. The SERS enhancement factor is approximately 102.
© (1999) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
PeiDi Bao, PeiDi Bao, XinMing Liu, XinMing Liu, TianQuan Huang, TianQuan Huang, Tieqiao Wu, Tieqiao Wu, Shiqui Liu, Shiqui Liu, "Surface-enhanced Raman scattering spectra of TvNPV polyhedrin protein in Ag sol", Proc. SPIE 3608, Biomedical Applications of Raman Spectroscopy, (9 April 1999); doi: 10.1117/12.345406; https://doi.org/10.1117/12.345406
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