US Army Tank-Automotive Command researchers are in the early stages of developing an autostereoscopic, 3D holographic visual display system. The present system uses holographic optics, low and high-resolution optics, low and high- resolution projectors, and computer workstation graphics to achieve real-time, 3D user-interactivity. This system is being used to conduct 3D visual perception studies for the purpose of understanding the effects of 3D in military target visual detection and as an alternative technique to CAD model visualization. The authors describe the present system configuration, operation, some of the technical limitations encountered during the system development, and the result of a human perception test that compared subject response times, hit rates and miss rates of visual detection when subjects used conventional 2D methods versus the 3D holographic image produced by the holographic display system. The results of this study revealed that 3D HOE system increased the perception of accuracy of moving vehicles. This research has provided some insights into which technology will be the best for presenting 3D simulated objects to subjects or designers in the laboratory.