The calibration procedure for a hot-wire anemometer system operating in a non-isothermal pressure-variant flow field is presented. Sensing of atmospheric velocity and temperature fluctuations from an altitude-variant platform using hot- wire anemometry equipment operating in both constant- temperature and constant-current modes requires calibration for velocity, temperature, and atmospheric pressure variations. Calibration tests to provide the range of velocity, temperature and pressure variations anticipated during Air Force Research Lab, Directed Energy Directorate- sponsored kite/tethered-balloon experiments were conducted and the result of these tests presented. The calibration tests were performed by placing the kite/tethered-balloon sensor package on a vehicle and driving from Kirtland AFB, NM to the top of Sandia Crest, a 10678 ft mountain range to the east of Albuquerque, NM. By varying the velocity of the van and conducting the test at different times of the day, variations in velocity, temperature and pressure within the range of those encountered during the kite/tethered-balloon experiments were obtained. The method of collapsing the calibration data is presented. Problems associated with collecting hot-wire anemometry data in a non-laboratory environment are discussed. Example data sets of temperature and velocity collected during the kite/tethered-balloon experiments are presented.