Detection of trinitrotoluene (TNT) extracted from soil using a surface plasmon resonance (SPR)-based sensor platform

Anita A. Strong; Donald I. Stimpson; Dwight U. Bartholomew; Thomas F. Jenkins; Jerome L. Elkind

doi:10.1117/12.357059

2 August 1999 Detection of trinitrotoluene (TNT) extracted from soil using a surface plasmon resonance (SPR)-based sensor platform

Anita A. Strong, Donald I. Stimpson, Dwight U. Bartholomew, Thomas F. Jenkins, Jerome L. Elkind

Author Affiliations +

Proceedings Volume 3710, Detection and Remediation Technologies for Mines and Minelike Targets IV; (1999) https://doi.org/10.1117/12.357059
Event: AeroSense '99, 1999, Orlando, FL, United States

Abstract

An antibody-based competition assay has been developed using a surface plasmon resonance (SPR) sensor platform for the detection of trinitrotoluene (TNT) in soil extract solutions. The objective of this work is to develop a sensor-based assay technology to use in the field for real- time detection of land mines. This immunoassay combines very simple bio-film attachment procedures and a low-cost SPR sensor design to detect TNT in soil extracts. The active bio-surface is a coating of bovine serum albumin that has been decorated with trinitrobenzene groups. A blind study on extracts from a large soil matrix was recently performed and result from this study will be presented. Potential interferant studied included 2,4-dinitrophenol, 2,4- dinitrotoluene, ammonium nitrate, and 2,4- dichlorophenoxyacetic acid. Cross-reactivity with dinitrotoluene will be discussed. Also, plans to reach sensitivity levels of 1ppb TNT in soil will be described.

Citation Download Citation

Anita A. Strong, Donald I. Stimpson, Dwight U. Bartholomew, Thomas F. Jenkins, and Jerome L. Elkind "Detection of trinitrotoluene (TNT) extracted from soil using a surface plasmon resonance (SPR)-based sensor platform", Proc. SPIE 3710, Detection and Remediation Technologies for Mines and Minelike Targets IV, (2 August 1999); https://doi.org/10.1117/12.357059

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