Time- and space-correlated single-photon counting system for fluorescence microspectroscopy of biological specimens

Mikhail R. Ainbund; Sergei Yu. Arzhantsev; Andrey Yuri Chikishev; Klaus Kemnitz; Nikolai I. Koroteev; Aleksandr P. Shkurinov; Bulat N. Toleutaev; Evgenii A. Turbin

doi:10.1117/12.340014

5 February 1999 Time- and space-correlated single-photon counting system for fluorescence microspectroscopy of biological specimens

Mikhail R. Ainbund, Sergei Yu. Arzhantsev, Andrey Yuri Chikishev, Klaus Kemnitz, Nikolai I. Koroteev, Aleksandr P. Shkurinov, Bulat N. Toleutaev, Evgenii A. Turbin

Author Affiliations +

Proceedings Volume 3732, ICONO '98: Laser Spectroscopy and Optical Diagnostics: Novel Trends and Applications in Laser Chemistry, Biophysics, and Biomedicine; (1999) https://doi.org/10.1117/12.340014
Event: ICONO '98: Laser Spectroscopy and Optical Diagnostics: Novel Trends and Applications in Laser Chemistry, Biophysics, and Biomedicine, 1998, Moscow, Russian Federation

Abstract

It is well known that fluorescence microspectroscopy is a powerful tool in the study of biological objects. Fluorescence lifetime imaging is one of the modern and promising techniques applied in these studies. The method implies the detection of not only spectral parameters of some molecules and molecular structures but also the fluorescence lifetimes. Thus, one can easily distinguish between two fluorophores with similar spectral properties but having different fluorescence lifetimes. Additional possibilities are offered by the method for stray light rejection and detection of Raman scattering because of the difference in time-correlation of the excitation, fluorescence, and Raman photons. We suggest the application of an original position-sensitive micro-channel plate photomultiplier as a key element of a newly developed fluorescence microspectrometer designated for the study of biological objects (cells, subcellular structures and organells). We present the first results of the experiments with the test objects. The results obtained clearly show that the new system is rather promising in the studies of living cells and tissues.

Citation Download Citation

Mikhail R. Ainbund, Sergei Yu. Arzhantsev, Andrey Yuri Chikishev, Klaus Kemnitz, Nikolai I. Koroteev, Aleksandr P. Shkurinov, Bulat N. Toleutaev, and Evgenii A. Turbin "Time- and space-correlated single-photon counting system for fluorescence microspectroscopy of biological specimens", Proc. SPIE 3732, ICONO '98: Laser Spectroscopy and Optical Diagnostics: Novel Trends and Applications in Laser Chemistry, Biophysics, and Biomedicine, (5 February 1999); https://doi.org/10.1117/12.340014

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available