Detection and identification of single molecules in microcapillaries

Christoph Zander; Karl-Heinz Drexhage

doi:10.1117/12.354800

19 July 1999 Detection and identification of single molecules in microcapillaries

Christoph Zander, Karl-Heinz Drexhage

Proceedings Volume 3749, 18th Congress of the International Commission for Optics; (1999) https://doi.org/10.1117/12.354800
Event: ICO XVIII 18th Congress of the International Commission for Optics, 1999, San Francisco, CA, United States

Abstract

A set-up permitting optical detection and identification of individual analyte molecules (dye labeled mononucleotides) in microcapillaries is described. It consists essentially of a confocal fluorescence microscope for time-correlated single-photon counting. Excitation of the analyte molecules takes place in the red spectral region with a short-pulsed diode laser at about 640 nm. A cone shaped capillary with an inner diameter at the very end of about 0.5 micrometers was used. The flow of the labeled nucleotides was established by electrokinetic forces. A maximum likelihood estimator was applied to analyze the measured fluorescence decays of the analyte molecules. This way two species of labeled mononucleotides can be distinguished by their characteristic fluorescence decay time on the single-molecule level.

Citation Download Citation

Christoph Zander and Karl-Heinz Drexhage "Detection and identification of single molecules in microcapillaries", Proc. SPIE 3749, 18th Congress of the International Commission for Optics, (19 July 1999); https://doi.org/10.1117/12.354800

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