19 July 1999 Detection and identification of single molecules in microcapillaries
Author Affiliations +
Proceedings Volume 3749, 18th Congress of the International Commission for Optics; (1999) https://doi.org/10.1117/12.354800
Event: ICO XVIII 18th Congress of the International Commission for Optics, 1999, San Francisco, CA, United States
Abstract
A set-up permitting optical detection and identification of individual analyte molecules (dye labeled mononucleotides) in microcapillaries is described. It consists essentially of a confocal fluorescence microscope for time-correlated single-photon counting. Excitation of the analyte molecules takes place in the red spectral region with a short-pulsed diode laser at about 640 nm. A cone shaped capillary with an inner diameter at the very end of about 0.5 micrometers was used. The flow of the labeled nucleotides was established by electrokinetic forces. A maximum likelihood estimator was applied to analyze the measured fluorescence decays of the analyte molecules. This way two species of labeled mononucleotides can be distinguished by their characteristic fluorescence decay time on the single-molecule level.
© (1999) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Christoph Zander, Christoph Zander, Karl-Heinz Drexhage, Karl-Heinz Drexhage, "Detection and identification of single molecules in microcapillaries", Proc. SPIE 3749, 18th Congress of the International Commission for Optics, (19 July 1999); doi: 10.1117/12.354800; https://doi.org/10.1117/12.354800
PROCEEDINGS
2 PAGES


SHARE
Back to Top