DNA-based fluorescent microarrays are fast becoming the preferred tool for studying a variety of complex biochemical phenomena ranging from multiplex mutation detection, to gene mapping and expression monitoring, and high throughput screening for new drug candidates. Fluorescence is a low energy phenomenon. The need for rapid, high resolution, wide field imaging of fluorescent microarrays calls for a specialized microscope architecture. We now describe the design of a 'Flying Objective' epi-fluorescence microscope that is ideally suited to this application, and compare the performance of this novel instrument with two other commercial epi-fluorescence microscopes designed to read DNA microarrays.
Jean I. Montagu,
"Fluorescence array scanner", Proc. SPIE 3779, Current Developments in Optical Design and Optical Engineering VIII, (5 October 1999); doi: 10.1117/12.368219; https://doi.org/10.1117/12.368219