Laboratory measurements of scattering by algae and comparison with Mie scattering

Jon Kare Lotsberg; Oyvind Frette; Svein Rune Erga; Jakob J. Stamnes

doi:10.1117/12.364188

16 September 1999 Laboratory measurements of scattering by algae and comparison with Mie scattering

Jon Kare Lotsberg, Oyvind Frette, Svein Rune Erga, Jakob J. Stamnes

Proceedings Volume 3821, Environmental Sensing and Applications; (1999) https://doi.org/10.1117/12.364188
Event: Industrial Lasers and Inspection (EUROPTO Series), 1999, Munich, Germany

Abstract

In this article we consider laboratory measurements of scattering by algae. We investigated the optical properties of a culture of the chlorophyte Chlamydomonas vectensis over almost two division cycles. The cell culture was synchronized which means that all cells were at the same stage in the growth cycle and thus divided at approximately the same time. By measuring the scattering properties of the synchronous culture during the full growth cycle, we get information about the influence of size changes of the individual cells as well as changes in the cell concentration. We measured of the scattering phase function in the interval from 1.7 degrees to 7.7 degrees at a wavelength of 633 nm using a CCD camera from Astrocam. In addition we measured the scattering phase function at 90 degrees, including the degree of polarization. We checked the experimental setup by doing a similar experiment in which we used monosized spheres from 'Dyno Particles AS,' so that the results can be compared with Mie calculations. The absorption and attenuation coefficients were measured by an AC9 instrument from WetLabs. The cell size was measured with a Coulter Counter, and the concentration was determined using a microscope. As expected, the optical properties were strongly dependent on the concentration of cells. Furthermore the shape of the scattering phase function and the degree of polarization were seen to depend not only on the concentration but also on the time of day.

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Jon Kare Lotsberg, Oyvind Frette, Svein Rune Erga, and Jakob J. Stamnes "Laboratory measurements of scattering by algae and comparison with Mie scattering", Proc. SPIE 3821, Environmental Sensing and Applications, (16 September 1999); https://doi.org/10.1117/12.364188

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KEYWORDS

Scattering

Mie scattering

Laser scattering

Light scattering

Refractive index

Scatter measurement

Polarization

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