Optical detection of polychlorinated biphenyls

Gabriela Kuncova; Daniela Berkova; Jiri Burkhard; Katerina Demnerova; Jarmila Pazlarova; Jan Triska; Nadezda Vrchotova

doi:10.1117/12.372879

21 December 1999 Optical detection of polychlorinated biphenyls

Gabriela Kuncova, Daniela Berkova, Jiri Burkhard, Katerina Demnerova, Jarmila Pazlarova, Jan Triska, Nadezda Vrchotova

Proceedings Volume 3853, Environmental Monitoring and Remediation Technologies II; (1999) https://doi.org/10.1117/12.372879
Event: Photonics East '99, 1999, Boston, MA, United States

Abstract

In this paper we describe the detection of polychlorinated biphenyls (PCBs) which is based on the measurement of changes of optical absorption at 400 nm of the medium in an aerobic bioreactor with immobilized cells Pseudomonas species 2. The rate of production, composition and the concentration of yellow intermediates are influenced by concentration and composition of PCB mixtures, concentration of cells and by the methods of immobilization. The method was applied in the detection of commercial mixture D103. It was found that the advantageous carriers were inorganic or organic-inorganic matrices, which sorbed PCBs and a cell outgrowth from their surface was low. In water contaminated with transformer oil and chlorinated hydrocarbons the detection limit is 10^-2 g_D103/kg. In transformer oil the upper limit for degradation of D103 by sodium dehalogenation (1.5 g_D103 /kg_oil) was determined also in the presence of the same concentration of trichloroethylene. The employment to of a liquid core waveguide spectrophotometer instead of a diode array spectrophotometer increased the sensitivity of the measurement of yellow intermediates by a factor of 100. An extrinsic fiber-optic sensor was used for in-situ measurement during biodegradation of PCBs in bioreactors.

Citation Download Citation

Gabriela Kuncova, Daniela Berkova, Jiri Burkhard, Katerina Demnerova, Jarmila Pazlarova, Jan Triska, and Nadezda Vrchotova "Optical detection of polychlorinated biphenyls", Proc. SPIE 3853, Environmental Monitoring and Remediation Technologies II, (21 December 1999); https://doi.org/10.1117/12.372879

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