15 December 1999 Integrated optical toxin sensor
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Abstract
We have developed a method for simple and highly sensitive detection of multivalent proteins using an optical waveguide sensor. The optical biosensor is based on optically tagged glycolipid receptors imbedded within a fluid phospholipid bilayer membrane formed on the surface of a planar optical waveguide. The binding of multivalent toxin initiates a fluorescence resonance energy transfer resulting in a distinctive spectral signature that is monitored by measuring emitted luminescence above the waveguide surface. The sensor methodology is highly sensitive and specific, and requires no additional reagents or washing steps. Demonstration of the utility of protein-receptor recognition using planar optical waveguides is shown here by the detection of cholera toxin.
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Dan Kelly, Xuedong Song, Daniel Keith Frayer, Sergio Brito Mendes, Nasser Peyghambarian, Basil I. Swanson, Karen M. Grace, "Integrated optical toxin sensor", Proc. SPIE 3858, Advanced Materials and Optical Systems for Chemical and Biological Detection, (15 December 1999); doi: 10.1117/12.372899; https://doi.org/10.1117/12.372899
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