15 December 1999 Two simple designs for surface-plasmon-resonance-based sensing
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Abstract
Surface plasmon resonance spectroscopy is becoming an increasingly important technique in biotechnology and chemical sensing. We present two simple, low cost, high sensitivity devices. The first is laser based mechanical implementation of a Kretschmann setup. Angle sweep is realized in two stages: step motor is used for coarse angle setting, and continuous angle sweep is achieved with a mirror on a floppy disk drive and a cylindrical lens setup. A single detector with AD converter defines resolution of the device through its sampling speed and dynamic range, so high sensitivity can be achieved. Sensor probes are metal- coated microscope slides and sample volume is temperature controlled. Second devices is a disposable cuvette for use in a VIS spectrometer. Specially designed monolithic polycarbonate block provides the required optical path and appropriate incidence angle on a thin metal film, deposited on the block. No equipment is necessary and the cuvette can be used within special cell, such as temperature controlled vessel. Device is also discussed in view of a low cost fiberoptic implementation. Some experimental results are presented to prove the applicability of devices. Disadvantages of technical solutions, used in devices, are also taken into consideration.
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Andrea Kobe, Andrea Kobe, Janez Ivan Mozina, Janez Ivan Mozina, "Two simple designs for surface-plasmon-resonance-based sensing", Proc. SPIE 3858, Advanced Materials and Optical Systems for Chemical and Biological Detection, (15 December 1999); doi: 10.1117/12.372902; https://doi.org/10.1117/12.372902
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