9 December 1999 Immobilization of a calcein derivative onto optical fibers for calcium ion sensing
Author Affiliations +
This paper describes a new fiber-optic calcium ion sensor using the calcein derivative, calcein acrylamide. Calcein acrylamide (CA) is a fluorescent indicator with a sensitivity for calcium ions at pH greater than 10. Ca2+ binds with CA with a binding ratio of 2:1 ratio therefore the concentration range for Ca2+ sensing depends on the amount of indicator present. Typically, calcium can be detected in the range 0 to 20 micrometers ol 1-1. The indicator can be covalently immobilized within a polymer matrix which itself is covalently immobilized onto a chemically modified optical fiber using photo-initiated polymerization. Three Ca2+ sensitive polymers are presented; CA in (1) poly-hydroxyethyl methacrylate (poly- HEMA), (2) poly-acrylamide and (3) poly-N-vinyl-pyrrolidinone (n-VP). The sensitivity of the immobilized CA are similar to that of CA in free solution.
© (1999) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
William D. Sloan, William D. Sloan, Mahesh Uttamlal, Mahesh Uttamlal, } "Immobilization of a calcein derivative onto optical fibers for calcium ion sensing", Proc. SPIE 3860, Fiber Optic Sensor Technology and Applications, (9 December 1999); doi: 10.1117/12.372991; https://doi.org/10.1117/12.372991

Back to Top