GFP fluorescence imaging with laser confocal scanning microscope

Yanhua Yu; Da Xing; Qiaojuan Shi; Junchu Zhou

doi:10.1117/12.364409

17 September 1999 GFP fluorescence imaging with laser confocal scanning microscope

Yanhua Yu, Da Xing, Qiaojuan Shi, Junchu Zhou

Proceedings Volume 3863, 1999 International Conference on Biomedical Optics; (1999) https://doi.org/10.1117/12.364409
Event: International Symposium on Biomedical Optics, 1999, Wuhan, China

Abstract

With gene marking technique, green fluorescent protein (GFP) and nodule bacteria gene has been linked together to form a single fusion gene expression vector. Then the vector is transferred into the nodule bacteria and the astragalus sinicus is invaded by the vector. With laser confocal scanning microscope, some important morphological information in plant nitrogen fixation research about the invading of nodule bacteria and the formation process of root nodule has been obtained through the 3D imaging of GFP reporting fluorescence.

Citation Download Citation

Yanhua Yu, Da Xing, Qiaojuan Shi, and Junchu Zhou "GFP fluorescence imaging with laser confocal scanning microscope", Proc. SPIE 3863, 1999 International Conference on Biomedical Optics, (17 September 1999); https://doi.org/10.1117/12.364409

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