Endovascular photodynamic therapy inhibits intimal hyperplasia after PTA in a rat model

Edward E. Gabeler M.D.; Richard van Hillegersberg; R. G. Statius van Eps; Wim Sluiter; Hero van Urk

doi:10.1117/12.386238

17 May 2000 Endovascular photodynamic therapy inhibits intimal hyperplasia after PTA in a rat model

Edward E. Gabeler M.D., Richard van Hillegersberg, R. G. Statius van Eps, Wim Sluiter, Hero van Urk

Proceedings Volume 3907, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems X; (2000) https://doi.org/10.1117/12.386238
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

Background: Vascular restenosis due to intimal hyperplasia (IH) and negative vascular remodeling (shrinkage) attenuates long-term patency of interventions like bypass procedures, endarterectomies and PT(C)As. Endovascular photodynamic therapy (PDT) may be an adjuvant approach based on intracellular photoactivation of 5-aminolevulinic acid (ALA) induced protoporphyrin IX (PP IX) resulting in local cytotoxicity. Aim: To prevent restenosis by inhibiting IH with endovascular PDT. Methods: Male Wistar rats (n equals 33) were subdivided into two groups; one experimental group; the PTA + PDT group (N equals 18), in which illumination with either 0, 12.5, 25 and 50 J/cm diffuser length was applied at 100 mW/cm diffuser length; one control group; the PTA group in which IH was induced after balloon denudation of the right iliacal artery (n equals 15). Results: Morfometric analysis showed that IH developed from 0.02 plus or minus 0.02 mm² at 1 week to 0.13 plus or minus 0.04 mm² at 4 weeks in the PTA group. In the PTA + PDT-group with 50 J/cm diffuser length still no IH (0.002 plus or minus 0.003 mm²) developed after 4 weeks. Conclusion: Endovascular PDT prevents formation of IH measured 4 weeks post PTA. An optimal light dosimetry of 50 J/cm diffuser length at 3 hours after ALA application was found in this IH model. Therefore, endovascular PDT is a promising therapy to prevent restenosis after vascular interventions.

Citation Download Citation

Edward E. Gabeler M.D., Richard van Hillegersberg, R. G. Statius van Eps, Wim Sluiter, and Hero van Urk "Endovascular photodynamic therapy inhibits intimal hyperplasia after PTA in a rat model", Proc. SPIE 3907, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems X, (17 May 2000); https://doi.org/10.1117/12.386238

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