Fluorescence detection of oral squamous cell carcinoma using Hyperflav

Ivan S. Melnik; Sergiy M. Dets; Andrew H. Rawicz; Lewei Zhang

doi:10.1117/12.386269

17 May 2000 Fluorescence detection of oral squamous cell carcinoma using Hyperflav

Ivan S. Melnik, Sergiy M. Dets, Andrew H. Rawicz, Lewei Zhang

Proceedings Volume 3907, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems X; (2000) https://doi.org/10.1117/12.386269
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

A novel hypericin-based drug Hyperflav^TM has been evaluated for light-induced fluorescence detection of oral cancer. Squamous cell carcinoma was induced with carcinogenic agent in right pouches of forty hamsters (20/20 males/females). Solution of Hyperflav^TM was sprinkled into stomach with a single dose 0.2 - 4 mg of pure hypericin per kg b.w. and 4 - 8 hours before fluorescence analysis. In two animal groups with cancer symptoms the autofluorescence and hypericin-induced fluorescence were taken under 442 nm excitation. The buccal mucosa and adjacent areas were measured fiberoptically in-vivo and in-vitro using orange/green ratio (610/540). The in-vivo fluorescence imaging of malignant areas was conducted to assist the biopsy guidance and to compare with white-light images. Histological and morphological analyses were performed from biopsies. Oral squamous cell carcinoma in its early stage demonstrated specific higher 610/540 ratio for 37 tested hamsters. Advanced state involved another higher fluorescence maximum around 640 nm that in our opinion caused by strong porphyrin-induced native fluorescence. Such deformation of fluorescence spectra may lead to inadequate perception of diseased tissue area. To avoid this problem the autofluorescence spectra & images were added. Hyperflav^TM application is promising for demarcation of early oral cancer when combined with autofluorescence measurements.

Citation Download Citation

Ivan S. Melnik, Sergiy M. Dets, Andrew H. Rawicz, and Lewei Zhang "Fluorescence detection of oral squamous cell carcinoma using Hyperflav", Proc. SPIE 3907, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems X, (17 May 2000); https://doi.org/10.1117/12.386269

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KEYWORDS

Luminescence

Cancer

Fluorescence spectroscopy

Tissue optics

In vivo imaging

Spectroscopy

Tissues

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