24 March 2000 Effect of diode laser radiation in root canal wall dentine: a microbiological study
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Proceedings Volume 3910, Lasers in Dentistry VI; (2000) https://doi.org/10.1117/12.380819
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States
Abstract
The aim of this study was to investigate the antibacterial effect of a diode laser in deep root canal dentine. The microbial colonization of root canal dentine can lead to failures in conventional endodontic treatment if only an inadequate bacterial reduction is achieved through canal treatment and chemical disinfection. 100 micrometer, 300 micrometer and 500 micrometer bovine dentine slices obtained by longitudinal sections were sterilized and inoculated on one side with an Enterococcus faecalis suspension. Laser radiation was performed on the opposite side with the diode laser, emits light at 810 nm and operates in the continuous wave mode (cw). Radiation was performed using a 400 micrometer tapered fiber tip at an angle of approx. 5 degrees to the surface over a period of 30 s. The output power at the distal end of the tip was 0.6 watt. The bacteria were then eluted through vibration and cultured on blood agar plates. The colony count reflected the antibacterial effect of laser radiation as a function of the layer thickness. A mean bacterial reduction of 74% was achieved even with a 500 micrometer thick slice. This investigation indicates that the diode laser can support the bacterial reduction in endodontic treatment.
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Norbert Gutknecht, Norbert Gutknecht, Georg Conrads, Georg Conrads, Christian Apel, Christian Apel, Claus Schubert, Claus Schubert, Friedrich Lampert, Friedrich Lampert, } "Effect of diode laser radiation in root canal wall dentine: a microbiological study", Proc. SPIE 3910, Lasers in Dentistry VI, (24 March 2000); doi: 10.1117/12.380819; https://doi.org/10.1117/12.380819
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