15 March 2000 Modular microinstrumentation for endothelial cell research
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Proceedings Volume 3912, Micro- and Nanotechnology for Biomedical and Environmental Applications; (2000) https://doi.org/10.1117/12.379565
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States
Abstract
Microfabrication technology is implemented to realize a fluidic microinstrument for the study of endothelial cell elongation and cell responsiveness to fluid flow. The microinstrument contains arrays of microchannels, 30 - 300 micrometer wide, that are fabricated by deep reactive ion etching (DRIE) of silicon and anodic bonding to glass. Silicon fluidic input/output modules, also micromachined in silicon, provide modular connections between the microchannels and off- chip devices for flow monitoring and control. Image analysis of cells cultured in microchannels shows that the cells become progressively more elongated as channel width decreases. When subjected to a fluid shear stress of 2 N/m2, cuboidal cells grown in 200 micrometer wide microchannels progressively align and elongate in the direction of flow.
© (2000) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Bonnie L. Gray, Abdul I. Barakat, Deborah K. Lieu, Scott D. Collins, Rosemary L. Smith, "Modular microinstrumentation for endothelial cell research", Proc. SPIE 3912, Micro- and Nanotechnology for Biomedical and Environmental Applications, (15 March 2000); doi: 10.1117/12.379565; https://doi.org/10.1117/12.379565
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