Immunoaffinity-based phosphorescent sensor platform for the detection of bacterial spores

Peter F. Scholl; C. Brent Bargeron; Terry E. Phillips; Tommy Wong; Sala Abubaker; John D. Groopman; Paul T. Strickland; Richard C. Benson

doi:10.1117/12.382033

11 April 2000 Immunoaffinity-based phosphorescent sensor platform for the detection of bacterial spores

Peter F. Scholl, C. Brent Bargeron, Terry E. Phillips, Tommy Wong, Sala Abubaker, John D. Groopman, Paul T. Strickland, Richard C. Benson

Author Affiliations +

Proceedings Volume 3913, In-Vitro Diagnostic Instrumentation; (2000) https://doi.org/10.1117/12.382033
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

Consideration of emergency response plans to an attack with biological weapons such as anthrax spores has spawned renewed interest in the development of inexpensive, rapid, and sensitive field portable sensors for use by non- specialists. The conceptual feasibility of such a device is demonstrated via the immunoaffinity capture of spores of the anthrax simulant B. globigii on a column followed by their washing, elution and phosphorescent detection. Spores are generically detected via the rapid extraction of dipicolinic acid (DPA) followed by its chelation with terbium to yield a phosphorescent complex. Chemical, thermal and mechanical methods of DPA extraction were evaluated. Simple extraction in HNO₃ released up to 5 percent of the spore weight as DPA within 60 seconds. Extraction in H₂O liberated 7 percent of the spore weight as DPA. Sonication with glass beads in H₂O for 45 seconds released up to 4 percent of the spore weight as DPA. It is estimated that implementation of these techniques will permit development of a device requiring 3-5 minutes per analysis with a limit of detection on the order of 500 ng spore/mL. This approach is not intended to replace more specific methods of analysis. However, it is proposed for consideration as an inexpensive, simple and rapid means of spore detection by non-specialists in emergency situations.

Citation Download Citation

Peter F. Scholl, C. Brent Bargeron, Terry E. Phillips, Tommy Wong, Sala Abubaker, John D. Groopman, Paul T. Strickland, and Richard C. Benson "Immunoaffinity-based phosphorescent sensor platform for the detection of bacterial spores", Proc. SPIE 3913, In-Vitro Diagnostic Instrumentation, (11 April 2000); https://doi.org/10.1117/12.382033

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