In-vitro measurements of light transmission parallel and perpendicular to the collagen orientation in tendons

Binod KC; Kevin R. Forrester; Dave Irvine-Halliday; Ken Muldrew; Cyril B. Frank; Nigel Shrive; Robert I. Thompson

doi:10.1117/12.382027

11 April 2000 In-vitro measurements of light transmission parallel and perpendicular to the collagen orientation in tendons

Binod KC, Kevin R. Forrester, Dave Irvine-Halliday, Ken Muldrew, Cyril B. Frank, Nigel Shrive, Robert I. Thompson

Author Affiliations +

Proceedings Volume 3913, In-Vitro Diagnostic Instrumentation; (2000) https://doi.org/10.1117/12.382027
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

Collimated light transmission studies of bovine tendon have been carried out to show the collagen waveguide effect. A monochromator/lamp apparatus was used to irradiate tissue samples and the transmitted light intensity was measured parallel and perpendicular to the collagen fiber orientation. The intensity parallel to the collagen fibers was at least twice that seen with perpendicular propagation. This indicates there is less scattering parallel to the fibers and light waveguiding is present for photon scattering. Also, absorption of light due to hemoglobin around 550 nm and due to water at 980nm was more prominent for parallel than perpendicular propagation. The light may travel either through the collagen fibril or fiber bundles or through the interstitial matrix. Sequential tests during tissue dehydration were performed and it was found that the transmitted light intensity increased with dehydration. This suggest that light may not be traveling through the interstitial matrix where water is the major component. Water may be acting as a reflection boundary for the light that is passing through the fibril or fiber bundles. Collagen waveguiding may be utilized to elucidate the collagen structure. Also, tissue water content could be measured from the transmission profiles. These may be of use in diagnosis and repair of connective tissues.

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Binod KC, Kevin R. Forrester, Dave Irvine-Halliday, Ken Muldrew, Cyril B. Frank, Nigel Shrive, and Robert I. Thompson "In-vitro measurements of light transmission parallel and perpendicular to the collagen orientation in tendons", Proc. SPIE 3913, In-Vitro Diagnostic Instrumentation, (11 April 2000); https://doi.org/10.1117/12.382027

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KEYWORDS

Tissues

Collagen

Tissue optics

Absorption

Light scattering

Waveguides

Scattering

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