11 April 2000 Multiphoton up-converting phosphors for use in rapid immunoassays
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Proceedings Volume 3913, In-Vitro Diagnostic Instrumentation; (2000) https://doi.org/10.1117/12.382032
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States
There exists increasing demand for rapid in-vitro diagnostic test capable of detecting pathogens, disease markers, and drugs. Many currently marketed technologies are limiting due to their analytical sensitivity or the number of analytes detected in a single assay. Presented is the use of up- converting phosphors as reporters in rapid point-of-care diagnostic assays using a lateral flow format in a nitrocellulose matrix. Up-converting Phosphor Technology (UPT) utilizes lanthanide-doped ceramic particles that possess anti-Stokes shift emission. These material absorb multiple IR photons and emit dopant-dependent spectra in the visible range. Their signature spectra allow the development of sensitive, multi-analyte diagnostic test. Because no biological matrix up-converts in the IR, the sensitivity of the assay is enhanced, being determined by the affinity of the biological constituents and the level of non-specific binding of the particle reporter. Feasibility data presented below represent a wide range of molecular weight analytes, including drugs of abuse, proteins such as cardiac markers, and pathogenic microorganisms.
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R. Sam Niedbala, Timothy L. Vail, Hans Feindt, S. Li, Jarrett L. Burton, "Multiphoton up-converting phosphors for use in rapid immunoassays", Proc. SPIE 3913, In-Vitro Diagnostic Instrumentation, (11 April 2000); doi: 10.1117/12.382032; https://doi.org/10.1117/12.382032

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