Photodynamic effect produced by HeNe radiation in Harderian glands of Wistar rats: an experimental model for PDT studies

Edmyr Rosa dos Reis; Ester Maria Danielli Nicola; Konradin Metze; Jorge Humberto Nicola

doi:10.1117/12.388091

13 June 2000 Photodynamic effect produced by HeNe radiation in Harderian glands of Wistar rats: an experimental model for PDT studies

Edmyr Rosa dos Reis, Ester Maria Danielli Nicola, Konradin Metze, Jorge Humberto Nicola

Author Affiliations +

Proceedings Volume 3914, Laser-Tissue Interaction XI: Photochemical, Photothermal, and Photomechanical; (2000) https://doi.org/10.1117/12.388091
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

In rats, the Harderian Gland secret Protoporphirin IX which is retained at acinar lumina. Since this photosensitizer is important for PDT of malignant tumors, we propose to study this gland as a model to help understanding PDT with endogenous photosensitizers. Twenty Wistar SPF adult rats were submitted to surgical exposure of both Harderian glands, revealing red fluorescence upon UV, characterizing the protoporphirin IX presence. After that, one gland of each pair (one kept as control) was irradiated with an 8 mW HeNe (6328 angstrom) for 45 minutes, delivering about 2.7 joules/mm². After 24 hours a group of 10 animals were scarified and the glands removed for histological analysis. The remaining animals were subjected to the same procedure but the glands were removed immediately after laser treatment. Histological and fluorescence analysis immediately after laser irradiation showed cell fragmentation with loss of acinar architecture with diffusion of protoporphirin in the cytoplasm of damaged cells, as well as interstitial edema. After 24 hours these alterations were more pronounced with accentuated loss of intraluminal protoporphirin and beginning of leukocytic demarcation of necrotic areas. The innate Harderian glands of rats, exposed to HeNe laser, showed a similar behavior as tumor tissue under PDT.

Citation Download Citation

Edmyr Rosa dos Reis, Ester Maria Danielli Nicola, Konradin Metze, and Jorge Humberto Nicola "Photodynamic effect produced by HeNe radiation in Harderian glands of Wistar rats: an experimental model for PDT studies", Proc. SPIE 3914, Laser-Tissue Interaction XI: Photochemical, Photothermal, and Photomechanical, (13 June 2000); https://doi.org/10.1117/12.388091

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